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KMID : 0613820150250030329
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Journal of Life Science
2015 Volume.25 No. 3 p.329 ~ p.335
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Optimization Mixture Ratio of Petasites japonicus, Luffa cylindrica and Houttuynia cordata to Develop a Functional Drink by Mixture Design
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Jeong Hae-Jin
Lee Kyoung-Pil
Chung Hun-Sik
Kim Dong-Seob
Kim Han-Soo
Choi Young-Whan
Im Dong-Soon
Seong Jong-Hwan
Lee Young-Guen
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Abstract
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This study was performed to determine the optimal ratio of Petasites japonicus, Luffa cylindrica, and Houttuynia cordata, all of which are supposed to have anti-respiratory disease effects, such as against rhinitis. The experiment incorporated a mixture design and included 12 experimental points with center replicates for three different independent variables (Petasites japonicus 30~70%; Luffa cylindrica 10~30%; and Houttuynia cordata 10~30%). Based on this design, the mixture was extracted in hot water at 121°C for 45 min and anti-allergy and anti-microbial activities were observed. The response surface and trace plot described for the anti-allergy activity showed Petasites japonicas was a relatively important factor. The correlation coefficient (R2) value 82.10% for the inhibition effect of degranulation was analyzed by the regression equation. The analysis of variance showed the model fit was statistically significant (p<0.05). The optimal ratio of the mixture was Petasites japonicus 0.75%, Luffa cylindrica 0.11%, and Houttuynia cordata 0.14%. The anti-microbial activity for each extraction of the mixture was valid on gram-positive, such as Staphylococcus aureus (KCCM 40881) and Staphylococcus epidermidis (KCCM 35494), while it was less effective on gram-negative, such as Escherichia coli (KCCM 11234) and Pseudomonas aeruginosa (KCCM 11328).
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KEYWORD
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Houttuynia cordata, Luffa cylindrical, mixture design, optimization, Petasites japonicas
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